PHR-GFP-STAT2
(Plasmid
#183931)
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PurposeOptogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherryN1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePHR-GFP-STAT2
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Alt namePHR domain of CRY2
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SpeciesA. thaliana (mustard weed), Synthetic
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Mutationnone
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Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHR-GFP-STAT2 was a gift from Karsten Rippe (Addgene plasmid # 183931 ; http://n2t.net/addgene:183931 ; RRID:Addgene_183931) -
For your References section:
Transcription activation is enhanced by multivalent interactions independent of phase separation. Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K. Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. 10.1016/j.molcel.2022.04.017 PubMed 35537448