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Addgene

NLS-dCas9-HA-NLS-GFP-VP16
(Plasmid #183923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-GFP-VP16
  • Alt name
    transactivation domain of UL48 (VP16)
  • Species
    Synthetic
  • Mutation
    none
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-dCas9-HA-NLS-GFP-VP16 was a gift from Karsten Rippe (Addgene plasmid # 183923 ; http://n2t.net/addgene:183923 ; RRID:Addgene_183923)

  • For your References section:

    Transcription activation is enhanced by multivalent interactions independent of phase separation. Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K. Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. 10.1016/j.molcel.2022.04.017 PubMed 35537448
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