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Addgene

GFP-LacI
(Plasmid #183920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1 (CMV replaced with SV2)
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-LacI
  • Species
    Synthetic
  • Mutation
    none
  • Entrez Gene
    lacI (a.k.a. O3K_19750)
  • Promoter SV2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BrGI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original pEYFP-C1 (Clontech) backbone CMV replaced by SV2 promoter and Color switch

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-LacI was a gift from Karsten Rippe (Addgene plasmid # 183920 ; http://n2t.net/addgene:183920 ; RRID:Addgene_183920)

  • For your References section:

    Transcription activation is enhanced by multivalent interactions independent of phase separation. Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K. Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 10.1016/j.molcel.2022.04.017. 10.1016/j.molcel.2022.04.017 PubMed 35537448
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