AbVec_IGKC
(Plasmid
#183702)
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PurposeEncode human Ig kappa chain constant region. For cloning variable region and mAb expression.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAbVec
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Backbone manufactureradapted from Burton lab
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIGKC
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Alt nameIgK constant region
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Insert Size (bp)500
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GenBank ID3514
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Entrez GeneIGKC (a.k.a. HCAK1, IGKCD, Km)
- Promoter SP6
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer SP6-F
- 3′ sequencing primer SV40-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byadapted from Burton lab mAb expression plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AbVec_IGKC was a gift from David Nemazee (Addgene plasmid # 183702 ; http://n2t.net/addgene:183702 ; RRID:Addgene_183702)
Map uploaded by the depositor.
