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Addgene

pDT-SuperFi-Cas9
(Plasmid #183630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMJ806
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 10577
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SuperFi-Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4107
  • Mutation
    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D
  • GenBank ID
    AAK33936.1 13622193
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original Cas9 gene was received from Addgene (pMJ806).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDT-SuperFi-Cas9 was a gift from David Taylor (Addgene plasmid # 183630 ; http://n2t.net/addgene:183630 ; RRID:Addgene_183630)

  • For your References section:

    Structural basis for mismatch surveillance by CRISPR-Cas9. Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW. Nature. 2022 Mar 2. pii: 10.1038/s41586-022-04470-1. doi: 10.1038/s41586-022-04470-1. 10.1038/s41586-022-04470-1 PubMed 35236982
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