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pcDNA pDEST40 Cas9-HMGB1
(Plasmid #183195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA pDEST40 Cas9 GW STOP
  • Backbone manufacturer
    Custom
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMGB1
  • Species
    Synthetic
  • Mutation
    n/a
  • Tag / Fusion Protein
    • Cas9WT (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA pDEST40 Cas9-HMGB1 was a gift from Emma Haapaniemi (Addgene plasmid # 183195 ; http://n2t.net/addgene:183195 ; RRID:Addgene_183195)

  • For your References section:

    Rapid genome editing by CRISPR-Cas9-POLD3 fusion. Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, Haapaniemi E. Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415. 10.7554/eLife.75415 PubMed 34898428
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