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Addgene

ePPE
(Plasmid #183095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJIT163
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nls-nCas9-NC-nls-MLV-Nls
  • Alt name
    nCas9-NC-MLV
  • Alt name
    Cas9 nickase
  • Species
    Synthetic
  • Mutation
    D10A in SpCas9

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ePPE was a gift from Caixia Gao (Addgene plasmid # 183095 ; http://n2t.net/addgene:183095 ; RRID:Addgene_183095)

  • For your References section:

    An engineered prime editor with enhanced editing efficiency in plants. Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao X, Gao C. Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w. 10.1038/s41587-022-01254-w PubMed 35332341
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