pcDNA3-RIIα-Dronpa
(Plasmid #182331)

• Purpose: PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
• Depositing Lab: Jin Zhang
• Publication: Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5412
• Total vector size (bp): 7308
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RIIalpha-Dronpa
• Species: H. sapiens (human)
• Insert Size (bp): 1896
• Entrez Gene: PRKAR2A (a.k.a. PKR2, PRKAR2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Full-length human PKA type 2alpha regulatory subunit (RIIalpha) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-RIIalpha-Dronpa.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry