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pDronpa-N1-Tau-μDpTT
(Plasmid #182323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDronpa-N1
  • Backbone size w/o insert (bp) 3984
  • Total vector size (bp) 6450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tau-μDronpa-TagRFP-T
  • Alt name
    Tau-Dronpa(H193T)-DEVD-TagRFP-T
  • Insert Size (bp)
    2466
  • Mutation
    Dronpa contains H193T mutation.
  • Promoter CMV
  • Tag / Fusion Protein
    • MAP tau (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer SV40pA reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDronpa-N1-Tau-μDpTT was a gift from Jin Zhang (Addgene plasmid # 182323 ; http://n2t.net/addgene:182323 ; RRID:Addgene_182323)

  • For your References section:

    DrFLINC Contextualizes Super-resolution Activity Imaging. Lin W, Mo GCH, Mehta S, Zhang J. J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13. 10.1021/jacs.1c05530 PubMed 34516108
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