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pCSC-ASCL1-IRES-GFP-T2A-Sox11
(Plasmid #182233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSC-SP-PW-IRES-GFP
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASCL1-IRES-GFP-T2A-Sox11
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    3300
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer agctcgtttagtgaaccgtcagatc
  • 3′ sequencing primer gagcaacatagttaagaataccag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSC-ASCL1-IRES-GFP-T2A-Sox11 was a gift from Chun-Li Zhang (Addgene plasmid # 182233 ; http://n2t.net/addgene:182233 ; RRID:Addgene_182233)

  • For your References section:

    Aging-relevant human basal forebrain cholinergic neurons as a cell model for Alzheimer's disease. Ma S, Zang T, Liu ML, Zhang CL. Mol Neurodegener. 2020 Oct 21;15(1):61. doi: 10.1186/s13024-020-00411-6. 10.1186/s13024-020-00411-6 PubMed 33087140
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