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Addgene

pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
(Plasmid #182049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-FLAG-IRES-blasticidin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox2
  • Species
    M. musculus (mouse)
  • Mutation
    S248A/T258A
  • Entrez Gene
    Sox2 (a.k.a. Sox-2, lcc, ysb)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A was a gift from Hyonchol Jang (Addgene plasmid # 182049 ; http://n2t.net/addgene:182049 ; RRID:Addgene_182049)

  • For your References section:

    O-GlcNAcylation of Sox2 at threonine 258 regulates the self-renewal and early cell fate of embryonic stem cells. Kim DK, Lee JS, Lee EY, Jang H, Han S, Kim HY, Hwang IY, Choi JW, Shin HM, You HJ, Youn HD, Jang H. Exp Mol Med. 2021 Nov;53(11):1759-1768. doi: 10.1038/s12276-021-00707-7. Epub 2021 Nov 24. 10.1038/s12276-021-00707-7 PubMed 34819616
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