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Addgene

MS2-pegRNA
(Plasmid #181811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    U6
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MS2-pegRNA
  • gRNA/shRNA sequence
    CACCTTCAGCTTGGCGGTCT

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MS2-pegRNA was a gift from Erik Sontheimer (Addgene plasmid # 181811 ; http://n2t.net/addgene:181811 ; RRID:Addgene_181811)

  • For your References section:

    A split prime editor with untethered reverse transcriptase and circular RNA template. Liu B, Dong X, Cheng H, Zheng C, Chen Z, Rodriguez TC, Liang SQ, Xue W, Sontheimer EJ. Nat Biotechnol. 2022 Apr 4. pii: 10.1038/s41587-022-01255-9. doi: 10.1038/s41587-022-01255-9. 10.1038/s41587-022-01255-9 PubMed 35379962
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