Lamp1-RFP
(Plasmid #1817)

• Purpose: Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
• Depositing Lab: Walther Mothes
• Publication: Sherer et al Traffic 2003 Nov;4(11):785-801.

Backbone

• Vector backbone: Modified Clontech Plasmid
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lysosome associated membrane protein 1
• Alt name: Lamp-1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1200
• Mutation: D50E (not important for function of plasmid)
• Entrez Gene: Lamp1 (a.k.a. LGP120)
• Tag / Fusion Protein:
  • RFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: DsRed1-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Norma Andrews provided Lamp-1
• Articles Citing this Plasmid:
  • 75 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1