pCDNA/golgin-160/myc
(Plasmid
#180632)
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PurposeExpression of WT golgin-160
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1
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Backbone manufacturerThermoFisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegolgin-160
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Alt nameGOLGA3
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Alt namegcp170
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SpeciesH. sapiens (human)
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Entrez GeneGOLGA3 (a.k.a. MEA-2, GCP170)
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Tag
/ Fusion Protein
- Myc-His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA/golgin-160/myc was a gift from Carolyn Machamer (Addgene plasmid # 180632 ; http://n2t.net/addgene:180632 ; RRID:Addgene_180632) -
For your References section:
The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information. Hicks SW, Machamer CE. J Biol Chem. 2002 Sep 27. 277(39):35833-9. 10.1074/jbc.M206280200 PubMed 12130652