pH3U3-zif268 (omega)
(Plasmid #18046)

• Depositing Lab: Scot Wolfe
• Publication: Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Backbone

• Vector backbone: pH3U3
• Backbone size w/o insert (bp): 5807
• Vector type: Bacterial Expression
• Selectable markers: URA3, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: zif268 binding site
• Insert Size (bp): 15
• Mutation: The preferred binding site for zif268 is inserted 10bp upstream of the -35box. This reporter plasmid is to be used as a positive test with pB1H2w2-zif268.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: HU100

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry