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Addgene

pmCherry-C-p50
(Plasmid #180238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 5928
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p50
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1206
  • Entrez Gene
    DCTN2 (a.k.a. DCTN50, DYNAMITIN, HEL-S-77, RBP50)
  • Tag / Fusion Protein
    • pmCherry-C1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer mCherry-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-C-p50 was a gift from Juan Bonifacino (Addgene plasmid # 180238 ; http://n2t.net/addgene:180238 ; RRID:Addgene_180238)

  • For your References section:

    Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon. Guo X, Farias GG, Mattera R, Bonifacino JS. Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):E5318-27. doi: 10.1073/pnas.1601844113. Epub 2016 Aug 24. 10.1073/pnas.1601844113 PubMed 27559088
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