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Anti-Mortalin/GRP75 [N52A/42.1R]
(Antibody #180091)

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Western Blot image by James Trimmer, UC Davis

Image 1 (of 3): Western Blot
Crude rat membranes immunoblotted against Anti-Mortalin/GRP75 hybridoma-derived antibody … View details
Credit: James Trimmer, UC Davis

Immunohistochemistry image by Zhuhao Wu, Weill Cornell Medicine

Image 2 (of 3): Immunohistochemistry
Delipidated mouse brain tissue was stained with Anti-Mortalin/GRP75 [N52A/42.1R]. View details
Credit: Zhuhao Wu, Weill Cornell Medicine

Immunohistochemistry image by

Image 3 (of 3): Immunohistochemistry
Free-floating rat brain sections were fixed and stained with purified Anti-Mortalin/GRP75… View details

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 180091-rAb 100 µg of purified recombinant antibody $250 *
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Recombinant Antibody trial size 180091-rAb.T 20 µg of purified recombinant antibody $85 *
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Features

Production & Usage

  • Production Plasmid
    Anti-Mortalin/GRP75 [N52A/42.1R]
  • Antigen Plasmid
    GRP75
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2750800

Terms and Licenses

Target Antigen

Protein Stress-70 protein, mitochondrial
Antigen Description Identified as off-target mAb in screen for anti-SALM2 NeuroMabs (immunogen was amino acids 551–766 of mouse SALM2). Target identified as Mortalin/GRP75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.
Species M. musculus (mouse)
Additional Species Reactivity H. sapiens (human),  R. norvegicus (rat),  C. elegans (nematode)
Gene Hspa9
Alternative Names
  • 75 kDa glucose-regulated protein
  • GRP-75
  • Heat shock 70 kDa protein 9
  • Mortalin
  • Peptide-binding protein 74
  • PBP74
  • p66 MOT
External References

Applications (4)

Information
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Immunohistochemistry

Recommended
1 image
Immunohistochemistry image by
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
1 image
Immunohistochemistry image by
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass
View Details

Western Blot

Recommended
1 image
Western Blot image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
2 images
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Mortalin/GRP75 [N52A/42.1R] - from James Trimmer (Addgene antibody # 180091 ; http://n2t.net/addgene:180091 ; RRID:AB_2750800)

  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360