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pCG_IFITM2-IRES_eGFP
(Plasmid #179959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFITM2
  • Species
    H. sapiens (human)
  • Mutation
    A33V, T41M, V121I
  • Entrez Gene
    IFITM2 (a.k.a. 1-8D, DSPA2c)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCG_IFITM2-IRES_eGFP was a gift from Frank Kirchhoff & Konstantin Sparrer (Addgene plasmid # 179959 ; http://n2t.net/addgene:179959 ; RRID:Addgene_179959)

  • For your References section:

    IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro. Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer KMJ, Kirchhoff F. Nat Commun. 2021 Jul 28;12(1):4584. 10.1038/s41467-021-24817-y PubMed 34321474
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