pCG_IFITM1-IRES_eGFP
(Plasmid
#179958)
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PurposeExpression vector for human IFITM1. Co-expresses GFP controlled by an IRES element.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIFITM1
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SpeciesH. sapiens (human)
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MutationP13A
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Entrez GeneIFITM1 (a.k.a. 9-27, CD225, DSPA2a, IFI17, LEU13)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG_IFITM1-IRES_eGFP was a gift from Frank Kirchhoff & Konstantin Sparrer (Addgene plasmid # 179958 ; http://n2t.net/addgene:179958 ; RRID:Addgene_179958) -
For your References section:
IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro. Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer KMJ, Kirchhoff F. Nat Commun. 2021 Jul 28;12(1):4584. 10.1038/s41467-021-24817-y PubMed 34321474