pGEX4T1-NDFIP2
(Plasmid
#179330)
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PurposeE. coli optimized expression of GST-fusion of human NDFIP2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX4T-1
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Backbone manufacturerGEHealthcare
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNDFIP2
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Alt nameN4WBP5A
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SpeciesH. sapiens (human)
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Insert Size (bp)728
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Entrez GeneNDFIP2 (a.k.a. N4WBP5A)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (not destroyed)
- 3′ cloning site Not1 (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T1-NDFIP2 was a gift from Arthur Haas (Addgene plasmid # 179330 ; http://n2t.net/addgene:179330 ; RRID:Addgene_179330)