pJS145
(Plasmid #179159)

• Purpose: Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
• Depositing Lab: Iva Greenwald
• Publication: Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;119(3). pii: 2117451119. doi: 10.1073/pnas.2117451119.

Backbone

• Vector backbone: pCFJ910 (pDESTR4-R3)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5496
• Total vector size (bp): 8109
• Vector type: Worm Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
• Species: C. elegans (nematode)
• Insert Size (bp): 2746
• Mutation: Flexon replacing 2nd intron of 2xNLS::GFP
• Promoter: rps-27p

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 Reverse
• 3′ sequencing primer: M13 Forward

Resource Information

• Supplemental Documents:
  • pJS145

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.452276v1 for bioRxiv preprint.