pFastBacMam5_N_Flag_His8_eGFP_3C
(Plasmid
#178816)
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Purpose(Empty Backbone) Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBac1
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Backbone manufacturerInvitrogen
- Backbone size (bp) 7446
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Modifications to backboneSynthetic DNA fragment containing CMV promoter, WPRE, and SV40 poly(A) signal was inserted into the vector for mammalian expression.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersGentamicin ; Tetracycline, Kanamycin
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Tag
/ Fusion Protein
- Flag, His8, eGFP, 3C (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBacMam5_N_Flag_His8_eGFP_3C was a gift from Kazuhiro Abe (Addgene plasmid # 178816 ; http://n2t.net/addgene:178816 ; RRID:Addgene_178816)
