pEF/myc/ER FLII12Pglu-700uDelta6
(Plasmid
#17867)
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEF/myc/ER
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameFLII12Pglu-700uDelta6
-
SpeciesE. coli
-
Insert Size (bp)2400
-
Mutation700uDelta6, ECFP insert in mglB
-
Tag
/ Fusion Protein
- Citrine (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEF/myc/ER FLII12Pglu-700uDelta6 was a gift from Wolf Frommer (Addgene plasmid # 17867 ; http://n2t.net/addgene:17867 ; RRID:Addgene_17867) -
For your References section:
GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Takanaga H, Chaudhuri B, Frommer WB. Biochim Biophys Acta. 2008 Apr . 1778(4):1091-9. 10.1016/j.bbamem.2007.11.015 PubMed 18177733
