pHAGE-UBC-TagRFP-T-ps
(Plasmid #178656)

• Purpose: Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells
• Depositing Lab: Jeffrey Gerst
• Publication: Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9873-E9882. doi: 10.1073/pnas.1706365114. Epub 2017 Oct 24.

Backbone

• Vector backbone: pHAGE-Ubc
• Backbone size w/o insert (bp): 7818
• Total vector size (bp): 8611
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TagRFP-T with palmitoylation signal at N-terminus
• Alt name: TagRFP-T-ps
• Species: Synthetic
• Insert Size (bp): 793
• GenBank ID: EU582019.1
• Promoter: Ubc
• Tag / Fusion Protein:
  • Palmitoylation signal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pHAGE-tagrfpt-PS.gbk
• A portion of this plasmid was derived from a plasmid made by: Cloned at Robert H. Singer lab (co-author on the paper)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry