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Addgene

pHAGE-UBC-TagRFP-T-ps
(Plasmid #178656)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHAGE-Ubc
  • Backbone size w/o insert (bp) 7818
  • Total vector size (bp) 8611
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TagRFP-T with palmitoylation signal at N-terminus
  • Alt name
    TagRFP-T-ps
  • Species
    Synthetic
  • Insert Size (bp)
    793
  • GenBank ID
    EU582019.1
  • Promoter Ubc
  • Tag / Fusion Protein
    • Palmitoylation signal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned at Robert H. Singer lab (co-author on the paper)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-UBC-TagRFP-T-ps was a gift from Jeffrey Gerst (Addgene plasmid # 178656 ; http://n2t.net/addgene:178656 ; RRID:Addgene_178656)
  • For your References section:

    Intercellular mRNA trafficking via membrane nanotube-like extensions in mammalian cells. Haimovich G, Ecker CM, Dunagin MC, Eggan E, Raj A, Gerst JE, Singer RH. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9873-E9882. doi: 10.1073/pnas.1706365114. Epub 2017 Oct 24. 10.1073/pnas.1706365114 PubMed 29078295