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pUltra-U6-gRNAs1-5
(Plasmid #178569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 178569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra
  • Backbone size w/o insert (bp) 8327
  • Total vector size (bp) 9423
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5 gRNA-tRNA array with U6 promoter
  • Insert Size (bp)
    1094

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see associated article for information on this vector's use in the context of the macsGESTALT lineage recording system: https://doi.org/10.1016/j.ccell.2021.05.005

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-U6-gRNAs1-5 was a gift from Christopher Lengner (Addgene plasmid # 178569 ; http://n2t.net/addgene:178569 ; RRID:Addgene_178569)

  • For your References section:

    Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states. Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ. Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10. 10.1016/j.ccell.2021.05.005 PubMed 34115987
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