pcDNA-gLuc-C1C2
(Plasmid
#178426)
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PurposeBioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA6.0 V5/His
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegLuc
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer caagggggccggtggtgacAAATGTGTCGAGCCACTGGGCATGG
- 3′ sequencing primer TGATGGTGATGATGACCGGTacagcccagcagctccaggcgc (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bygLuc was a gift from Contag lab; C1C2 domain is from Agnese Mariotti Addgene, plasmid # 46830
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-gLuc-C1C2 was a gift from Masako Harada (Addgene plasmid # 178426 ; http://n2t.net/addgene:178426 ; RRID:Addgene_178426) -
For your References section:
Engineering Extracellular Vesicles to Target Pancreatic Tissue In Vivo. Komuro H, Kawai-Harada Y, Aminova S, Pascual N, Malik A, Contag CH, Harada M. Nanotheranostics. 2021 Apr 15;5(4):378-390. doi: 10.7150/ntno.54879. eCollection 2021. 10.7150/ntno.54879 PubMed 33912378