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Addgene

scAAV-sgRNA-GFP
(Plasmid #177935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    scAAV vector
  • Vector type
    Mammalian Expression, AAV, CRISPR
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses EGFP marker

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    scAAV-sgRNA-GFP was a gift from Roland Rad (Addgene plasmid # 177935 ; http://n2t.net/addgene:177935 ; RRID:Addgene_177935)

  • For your References section:

    CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver. Kaltenbacher T, Loprich J, Maresch R, Weber J, Muller S, Oellinger R, Gross N, Griger J, de Andrade Kratzig N, Avramopoulos P, Ramanujam D, Brummer S, Widholz SA, Barthel S, Falcomata C, Pfaus A, Alnatsha A, Mayerle J, Schmidt-Supprian M, Reichert M, Schneider G, Ehmer U, Braun CJ, Saur D, Engelhardt S, Rad R. Nat Protoc. 2022 Apr;17(4):1142-1188. doi: 10.1038/s41596-021-00677-0. Epub 2022 Mar 14. 10.1038/s41596-021-00677-0 PubMed 35288718
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