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Addgene

pYL-kasOp-FnCas12a3
(Plasmid #177875)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177875 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRISPomyces-2
  • Vector type
    CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptomyces codon optimized the FnCas12a mutant EP16
  • Alt name
    EP16
  • Species
    Streptomyces
  • Insert Size (bp)
    3909
  • Promoter kasOp*

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCGAACAGGCCATTCACAGA
  • 3′ sequencing primer TCTGACGCTCAGTGGAACGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYL-kasOp-FnCas12a3 was a gift from Yunzi Luo (Addgene plasmid # 177875 ; http://n2t.net/addgene:177875 ; RRID:Addgene_177875)

  • For your References section:

    Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems. Zhang J, Zhang D, Zhu J, Liu H, Liang S, Luo Y. Front Bioeng Biotechnol. 2020 Jun 30;8:726. doi: 10.3389/fbioe.2020.00726. eCollection 2020. 10.3389/fbioe.2020.00726 PubMed 32695773
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