p403 – TDH3-MTS-mCherry-GFP1-10-His
(Plasmid
#177730)
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PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep403 TDH3
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-GFP1-10
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Tag
/ Fusion Protein
- Mitochondrial Targeting Sequence (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p403 – TDH3-MTS-mCherry-GFP1-10-His was a gift from Erik Böttger (Addgene plasmid # 177730 ; http://n2t.net/addgene:177730 ; RRID:Addgene_177730) -
For your References section:
ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function. Cortes Sanchon A, Santhosh Kumar H, Mantovani M, Osinnii I, Mateos JM, Kaech A, Shcherbakov D, Akbergenov R, Bottger EC. Commun Biol. 2021 Dec 2;4(1):1350. doi: 10.1038/s42003-021-02873-w. 10.1038/s42003-021-02873-w PubMed 34857875
