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Addgene

pCLNR-H2BG
(Plasmid #17735)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLNRX
  • Backbone size w/o insert (bp) 7900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-GFP
  • Alt name
    Human histone H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • GenBank ID
    X00088
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ClaI (destroyed during cloning)
  • 5′ sequencing primer pREP-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLNR-H2BG was a gift from Geoff Wahl (Addgene plasmid # 17735 ; http://n2t.net/addgene:17735 ; RRID:Addgene_17735)

  • For your References section:

    Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Kanda T, Sullivan KF, Wahl GM. Curr Biol. 1998 Mar 26. 8(7):377-85. 10.1016/S0960-9822(98)70156-3 PubMed 9545195
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