bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st
(Plasmid
#177232)
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PurposeExpresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL3.3
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgSik1_1st/sgSik2/sgSik3_1st
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gRNA/shRNA sequenceGATCATGTCGGAGTTCAGTG/TGATACAGTTTAATGATGTG/TGGCAGAGAAGGAAGCTCGA
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SpeciesM. musculus (mouse)
- Promoter bU6/mU6/hU6
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st was a gift from Monte Winslow (Addgene plasmid # 177232 ; http://n2t.net/addgene:177232 ; RRID:Addgene_177232)
