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Addgene

pSUC01
(Plasmid #177204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSET152
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7959
  • Modifications to backbone
    the integrase gene int phiC31 was removed
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    none
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SaindC
  • Alt name
    indS
  • Species
    Streptomyces albidoflavus
  • Insert Size (bp)
    3927
  • Mutation
    NONE
  • GenBank ID
  • Promoter TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTGTAAAGTCGTGGCCAGGAGAATACGACAGCGTGCAGGACTGGGGGAGTT
  • Tag / Fusion Protein
    • NONE

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gaatcttcgctgaagtc
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUC01 was a gift from Wenjun Guan (Addgene plasmid # 177204 ; http://n2t.net/addgene:177204 ; RRID:Addgene_177204)

  • For your References section:

    An Efficient Markerless Deletion System Suitable for the Industrial Strains of Streptomyces. Dong J, Wei J, Li H, Zhao S, Guan W. J Microbiol Biotechnol. 2021 Dec 28;31(12):1722-1731. doi: 10.4014/jmb.2106.06083. 10.4014/jmb.2106.06083 PubMed 34489377
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