pRS306Sup35R1
(Plasmid #17715)

• Depositing Lab: Susan Lindquist
• Publication: Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-12.

Backbone

• Vector backbone: pRS306
• Backbone size w/o insert (bp): 4300
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pRS306Sup35R1
• Alt name: sup35
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3300
• Mutation: An pRS306 based integrating plasmid designed for cloning repeats in Sup35. The last four Sup35 repeats have been removed, and there is a region to clone repeats in BspE1 and Eag1 sites into this location.
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: SacI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry