pGEX4T3-GST-Tev
(Plasmid #177142)

• Purpose: Bacterial vector for expression of an N-terminal GST fusion of the Tev protease
• Depositing Lab: Robert Sobol
• Publication: Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):6269-6286. doi: 10.1093/nar/gkz293.

Backbone

• Vector backbone: pGEX4T3
• Backbone manufacturer: Sigma
• Total vector size (bp): 5675
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: use BL21-Codonplus-RP for protein expression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tev
• Species: Virus
• Insert Size (bp): 729
• Promoter: Tac
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CCGCGTGGATCCCATGGAGAAAGCTTGTTTAAGG
• 3′ sequencing primer: CACGATGCGGCCGCTTAGCGACGGCGACGACGATTC

Resource Information

• Supplemental Documents:
  • pGEX4T3-GST-Tev.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry