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Addgene

pcR295/527
(Plasmid #17679)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVX
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    c-src K295R Y527F
  • Alt name
    c-Src
  • Alt name
    src
  • Species
    G. gallus (chicken)
  • Mutation
    Lys 295 changed to Arg. Tyr 527 changed to Phe.
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Based on plasmid pM5HHB5. This plasmid is unpublished, but related to this article.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcR295/527 was a gift from David Shalloway (Addgene plasmid # 17679 ; http://n2t.net/addgene:17679 ; RRID:Addgene_17679)

  • For your References section:

    Altered tyrosine 527 phosphorylation and mitotic activation of p60c-src. Bagrodia S, Chackalaparampil I, Kmiecik TE, Shalloway D. Nature. 1991 Jan 10. 349(6305):172-5. 10.1038/349172a0 PubMed 1702522
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