pcR295
(Plasmid #17678)

• Depositing Lab: David Shalloway
• Publication: Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Backbone

• Vector backbone: pEVX
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: c-src K295R
• Alt name: c-Src
• Alt name: src
• Species: G. gallus (chicken)
• Mutation: Lys 295 changed to Arg.
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on plasmid pM5HHB5.