pM5HHB5
(Plasmid
#17674)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEVX
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namec-src
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Alt namec-Src
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Alt namesrc
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SpeciesG. gallus (chicken)
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Entrez GeneSRC (a.k.a. PP60C-SCR, SDR, c-src)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid contains a c-src gene that encodes normal chicken pp60 c-src flanked by MoMLV LTRs from expression vector pEVX.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM5HHB5 was a gift from David Shalloway (Addgene plasmid # 17674 ; http://n2t.net/addgene:17674 ; RRID:Addgene_17674) -
For your References section:
Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation. Kmiecik TE, Shalloway D. Cell. 1987 Apr 10. 49(1):65-73. 10.1016/0092-8674(87)90756-2 PubMed 3103925
Map uploaded by the depositor.
