pVPS13D-I-EGFP
(Plasmid
#176464)
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Purposeexpress internally GFP-tagged VPS13D in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3679
- Total vector size (bp) 17786
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVPS13D
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Alt nameSCAR4
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SpeciesH. sapiens (human)
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Insert Size (bp)11871
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MutationEYFP is internally inserted
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GenBank IDNM_015378 ENSG00000048707
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Entrez GeneVPS13D (a.k.a. BLTP5D, SCAR4)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP-Ub
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGAAGCGCGATCACATGG
- 3′ sequencing primer CGGTGAACAGCTCCTCGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EYFP is internally inserted to replace the alternative skipping exon 40 of VPS13D (in-frame)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVPS13D-I-EGFP was a gift from Richard Youle (Addgene plasmid # 176464 ; http://n2t.net/addgene:176464 ; RRID:Addgene_176464) -
For your References section:
VPS13D promotes peroxisome biogenesis. Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, Prinz WA, Youle RJ. J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 10.1083/jcb.202001188. 10.1083/jcb.202001188 PubMed 33891012