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Addgene

pLX304-IRES-Td
(Plasmid #176296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone manufacturer
    None
  • Backbone size (bp) 10000
  • Modifications to backbone
    an IRES-TdTomato cassette was sub cloned into the NheI-AgeI sites
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter CMV
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Longer growth may be needed
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-IRES-Td was a gift from William Kaelin (Addgene plasmid # 176296 ; http://n2t.net/addgene:176296 ; RRID:Addgene_176296)

  • For your References section:

    Targeting oncoproteins with a positive selection assay for protein degraders. Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr. Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb. 10.1126/sciadv.abd6263 PubMed 33547076
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