pLX304-IRES-GFP
(Plasmid #176288)

• Purpose: (Empty Backbone) pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites
• Depositing Lab: William Kaelin
• Publication: Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb.

Backbone

• Vector backbone: pLX304
• Backbone manufacturer: None
• Backbone size (bp): 10000
• Modifications to backbone: an IRES-GFP cassette was sub cloned into the NheI-AgeI site
• Vector type: Mammalian Expression, Lentiviral
• Promoter: CMV
• Selectable markers: Blasticidin
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Longer growth may be needed
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: attB2

Resource Information

• Supplemental Documents:
  • MAPpLX304-gate-IRES-GFP copy.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry