EF.hICN1.CMV.GFP
(Plasmid #17623)

• Purpose:
• Depositing Lab: Linzhao Cheng
• Publication: Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Backbone

• Vector backbone: EF.v-CMV.GFP
• Backbone manufacturer: Linzhao Cheng Lab
• Backbone size w/o insert (bp): 8200
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ICN1
• Alt name: intracellular domain of the Notch receptor
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.

The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.