EF.mHES1.CMV.GFP
(Plasmid #17622)

• Depositing Lab: Linzhao Cheng
• Publication: Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Backbone

• Vector backbone: EF.v-CMV.GFP
• Backbone manufacturer: Linzhao Cheng Lab
• Backbone size w/o insert (bp): 8200
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HES1
• Alt name: mHES1
• Alt name: Hes-1
• Species: M. musculus (mouse)
• Insert Size (bp): 850
• Entrez Gene: Hes1 (a.k.a. Hry, bHLHb39)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. mHES1 was cloned downstream of the EF-1a promoter.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.