EF.PGK.GFP
(Plasmid #17618)

• Purpose: (Empty Backbone)
• Depositing Lab: Linzhao Cheng
• Publication: Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Backbone

• Vector backbone: EF.PGK.GFP
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a forward

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In a SIN LV containing the elongation factor 1alpha promoter, we included a PGK promoter that drives expression of GFP as a reporter. Dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell.

Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.