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Addgene

dimericTomato-1xwGBD
(Plasmid #176099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WAS
  • Species
    H. sapiens (human)
  • Entrez Gene
    WAS (a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
  • Promoter CMVdel

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dimericTomato-1xwGBD was a gift from Dorus Gadella (Addgene plasmid # 176099 ; http://n2t.net/addgene:176099 ; RRID:Addgene_176099)

  • For your References section:

    Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, Goedhart J. J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. Epub 2023 May 25. 10.1242/jcs.260802 PubMed 37226883
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