Human IFN-gamma promoter/enhancer
(Plasmid #17598)

• Depositing Lab: Howard Young
• Publication: Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IFN gamma promoter with 1st intron enhancer
• Alt name: -3.6kb IFN-gamma luc - kB STAT
• Alt name: interferon gamma
• Species: H. sapiens (human)
• Insert Size (bp): 4300
• Mutation: -3.6 kb to +70 of promoter plus 680 bp of IFN-gamma 1st intron inserted downstream of luciferase
• Entrez Gene: IFNG (a.k.a. IFG, IFI, IMD69)
• Tag / Fusion Protein:
  • luciferase

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Young lab stock #D3314.
SalI should give 8.6kb + 0.6kb bands.