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pEF6 VPS51-V5-6His
(Plasmid #175502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6-V5-His
  • Backbone size w/o insert (bp) 5840
  • Total vector size (bp) 8186
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS51
  • Alt name
    ANG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2346
  • Mutation
    A661V- please see depositor comments
  • Entrez Gene
    VPS51 (a.k.a. ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms A661V mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6 VPS51-V5-6His was a gift from Juan Bonifacino (Addgene plasmid # 175502 ; http://n2t.net/addgene:175502 ; RRID:Addgene_175502)

  • For your References section:

    Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex. Perez-Victoria FJ, Schindler C, Magadan JG, Mardones GA, Delevoye C, Romao M, Raposo G, Bonifacino JS. Mol Biol Cell. 2010 Oct 1;21(19):3386-95. doi: 10.1091/mbc.E10-05-0392. Epub 2010 Aug 4. 10.1091/mbc.E10-05-0392 PubMed 20685960
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