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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCoHYGRO
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Backbone manufacturerInvitrogen
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Vector typeInsect Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePuromycine Acetyl Transferase
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Alt namepuromycin N-acetyl-transferase
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Insert Size (bp)600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byStratagene pExchange module EC-Puro
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCoPURO was a gift from Francis Castellino (Addgene plasmid # 17533 ; http://n2t.net/addgene:17533 ; RRID:Addgene_17533) -
For your References section:
Rapid selection of Drosophila S2 cells with the puromycin resistance gene. Iwaki T, Figuera M, Ploplis VA, Castellino FJ. Biotechniques. 2003 Sep . 35(3):482-4, 486. 10.2144/03353bm08 PubMed 14513552