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pcDNA3.1(+)_Pmel-1_TCRB
(Plasmid #175288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6318
  • Vector type
    Mammalian Expression ; Suitable for IVT
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T cell receptor beta chain
  • Alt name
    T cell receptor beta chain mRNA, complete cds
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    954
  • GenBank ID
    EF154514.1
  • Entrez Gene
    Tcrb (a.k.a. TCRbeta, Tib)
  • Promoter CMV and T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The vector was from Genscript. The insert sequence was taken from GenBank (EF154514.1). Reference cited below in comments:

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Overwijk, Willem W., Marc R. Theoret, Steven E. Finkelstein, Deborah R. Surman, Laurina A. de Jong, Florry A. Vyth-Dreese, Trees A. Dellemijn, et al. 2003. “Tumor Regression and Autoimmunity after Reversal of a Functionally Tolerant State of Self-Reactive CD8+ T Cells.” The Journal of Experimental Medicine 198 (4): 569–80.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_Pmel-1_TCRB was a gift from Jeff Hammerbacher (Addgene plasmid # 175288 ; http://n2t.net/addgene:175288 ; RRID:Addgene_175288)

  • For your References section:

    Discovering tumor-reactive T-cell receptors through single-cell sequencing of tumor-infiltrating lymphocytes. Gottschalk E, Aksoy BA, Aksoy P, Swiderska-syn M, Mart C, Macpherson L, Romeo M, Smith A, Knochelmann H, Paulos C, Timmers C, Wrangle J, Rubinstein MP, Hammerbacher J. bioRxiv 2021.11.30.470597 10.1101/2021.11.30.470597
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