pRSET iGluSnFR3 v857
(Plasmid #175186)

• Purpose: Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.
• Depositing Lab: Kaspar Podgorski
• Publication: Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 2863
• Total vector size (bp): 4429
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluSnFR3 v857
• Alt name: 556dot857
• Alt name: iGluSnFR3 v857
• Alt name: iGluSnFR3
• Species: Synthetic
• Insert Size (bp): 1566
• Promoter: T7
• Tag / Fusion Protein:
  • His tag, T7 leader, Xpress epitope (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGGG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pRSET iGluSnFR3 v857 genbank.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry