pLV Nup62-V5
(Plasmid
#175116)
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PurposeLentiviral expression of mouse Nup62-V5
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Backbone manufacturerTobias Meyer (Addgene plasmid # 85132)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup62
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SpeciesM. musculus (mouse)
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Entrez GeneNup62 (a.k.a. D7Ertd649e, Nupc1, p62)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
G113V and A319V point mutations are natural sequence variations in Nup62
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV Nup62-V5 was a gift from Larry Gerace (Addgene plasmid # 175116 ; http://n2t.net/addgene:175116 ; RRID:Addgene_175116)